Coding

Part:BBa_K3809001

Designed by: Francisco Javier Castañeda Villagran   Group: iGEM21_TecCEM   (2021-08-13)


Novel Selection Cassette katE

The part was desinged to create a novel selection marker for a modified strain of E. coli without the genes involved in the methabolic pathway of glycerol and peroxide. It codes for the E. coli catalase gene katE which converts peroxide into water and oxygen. It may be used for other purposes not stated here.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1842
    Illegal BamHI site found at 1752
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 111
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None